发明名称 |
Over-expression of enzymes involved in post-translational protein modifications in human cells |
摘要 |
Methods for producing and/or propagating virus particles that are present in a virus isolate obtained from an infected subject by contacting a host cell with a virus particle and culturing the cell under conditions conducive to propagation of the virus particle are disclosed. A method for selective propagation of a set of virus particles which have an affinity for receptors comprising a specific glycosylation residue are further disclosed. Immortalized human embryonic retina cells comprising a nucleic acid sequence encoding an adenoviral E1A protein integrated into the genome of the cells and a nucleic acid sequence encoding an enzyme involved in post-translational modification of proteins, wherein said nucleic acid sequence encoding the enzyme involved in post-translational modification of proteins is under control of a heterologous promoter are further disclosed. Methods for production of recombinant proteins from such cells and obtaining such recombinant proteins having increased sialylation are also described.
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申请公布号 |
US2007231860(A1) |
申请公布日期 |
2007.10.04 |
申请号 |
US20070731246 |
申请日期 |
2007.03.30 |
申请人 |
CRUCELL HOLLAND B.V. |
发明人 |
UYTDEHAAG ALPHONSUS G.;OPSTELTEN DIRK J. |
分类号 |
C12P1/04;C07K14/075;C07K14/11;C07K14/16;C12N15/861 |
主分类号 |
C12P1/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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