摘要 |
A method is disclosed for separating selected cells that have cell membranes which are selectively-permeable to a given solute from other non-selected cells. The combination of cells are exposed to the given solute and thereafter the cells are exposed to low or zero-solute solution to thereby osmotically shock and destroy non-selected cells. Solute examples are ions, sugars, amino acids or other permeant particles. Cells which may be selected for isolation by glucose or a glucose substitute are islets, neurons, incretin secreting cells and cells transfected with the GLUT 2 gene. |