METHOD OF PRODUCING BIOLOGICALLY ACTIVE VITAMIN K DEPENDENT PROTEINS BY RECOMBINANT METHODS
摘要
The invention relates to commercially viable methods for producing biologically active vitamin K dependent proteins, particularly Factor IX. Factor IX is produced at a level of at least about 15 mg/L and is at least 25% biologically active. The method relies upon co-expression of one or more of paired basic amino acid converting enzyme (PACE), vitamin K dependent epoxide reductase (VKOR) and vitamin K dependent ?-glutamyl carboxylase (VKGC) at a preferred ratio so that the vitamin K dependent protein is efficiently produced and processed by a recombinant cell.
申请公布号
WO2007075976(A3)
申请公布日期
2007.10.04
申请号
WO2006US48954
申请日期
2006.12.21
申请人
INSPIRATION BIOPHARMACEUTICALS;UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL;DROHAN, WILLIAM, N.;GRIFFITH, MICHAEL, J.;TAYLOR, JOHN, R.;STAFFORD, DARREL, W.
发明人
DROHAN, WILLIAM, N.;GRIFFITH, MICHAEL, J.;TAYLOR, JOHN, R.;STAFFORD, DARREL, W.