REACTIVE OXYGEN DETERMINATION REAGENT

摘要

Disclosed is a compound represented by the general formula (I) or a salt thereof: (I) wherein R<SUP>1</SUP> and R<SUP>2</SUP> independently represent a hydrogen atom, a C<SUB>1-6</SUB> alkyl group, or a group represented by the formula (A), provided that at least one of R<SUP>1</SUP> and R<SUP>2</SUP> represents the group represented by the formula (A): (A) wherein X<SUP>1</SUP> represents -N(R<SUP>3</SUP>)(R<SUP>4</SUP>) (wherein R<SUP>3</SUP> and R<SUP>4</SUP> independently represent a hydrogen atom or a C<SUB>1-6</SUB> alkyl group), a hydroxy group, a C<SUB>1-6</SUB> alkoxy group, a sulfanyl group or an alkylsulfanyl group; X<SUP>2</SUP> represents a hydrogen atom or one to three univalent substituents substituting on the benzene ring; Y represents -O- or -S-; and n is an integer of 1 to 6. The compound can serve as a substrate for luciferase and is useful as a reactive oxygen determination reagent Also disclosed is a reagent for use in the determination of reactive oxygen, which comprises the compound.