发明名称 |
MICROINJECTION PROCEDURE FOR GENE TRANSFER IN FISH |
摘要 |
A method is provided for transferring into a fish egg DNA of at least one gene foreign to the fish egg so as to integrate the foreign DNA stably into the fish egg genome. The method comprises: inserting a hollow needle into the fish egg, through the micropyle thereof, and injecting therethrough and into the fish egg a selected volume of a solution containing a plurality of copies of the foreign DNA. The fish egg may be fertilized and in the inactive state during the injection procedure and activated after injection is completed. The method is particularly suited to transferring freeze-resistant characteristics of icy water fish to salmonids.
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申请公布号 |
CA1341553(C) |
申请公布日期 |
2007.10.02 |
申请号 |
CA19880584435 |
申请日期 |
1988.11.29 |
申请人 |
HSC RESEARCH DEVELOPMENT CORPORATION;QUEENS UNIVERSITY AT KINGSTON;SEABRIGHT CORPORATION LTD |
发明人 |
HEW, CHOY LEONG;DAVIES, PETER L.;FLETCHER, GARTH LEONARDO |
分类号 |
C12N15/89;C12N15/90;C12N5/10;C12N5/06 |
主分类号 |
C12N15/89 |
代理机构 |
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