发明名称 |
CLASS I SEQUENCE BASED TYPING OF HLA-A, -B, AND -C ALLELES BY DIRECT DNA SEQUENCING |
摘要 |
<p>There is provided a method for directly typing or sequencing HLA-A, -B, or - C alleles from a tissue sample wherein exons 2 and 3 of the HLA-A, -B, or -C alleles from the sample are amplified together in a locus specific manner and then separated out and individually amplified in a locus specific manner. After the two amplifications, the amplified exons are directly sequenced, the sequences are recombined, and a comparison is made between the derived HLA allele sequence and an HLA allele database, thereby giving an exact HLA-A, -B, or -C type for the sample being tested.</p> |
申请公布号 |
CA2280156(C) |
申请公布日期 |
2007.10.02 |
申请号 |
CA19982280156 |
申请日期 |
1998.02.11 |
申请人 |
HILDEBRAND, WILLIAM H.;DUTHIE, R. SCOTT;CHRETIEN, PIERRE;ELLEXSON, MARY |
发明人 |
HILDEBRAND, WILLIAM H.;DUTHIE, R. SCOTT;CHRETIEN, PIERRE;ELLEXSON, MARY |
分类号 |
C12Q1/68;C12N15/12;C12P19/34 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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