<p>To provide a biosensor exhibiting high storage stability in which variations in measurements due to storage temperature are extremely small, and to provide a manufacturing method of the biosensor. A method for manufacturing a biosensor (10) comprises a reactive portion forming step which comprises a first layer forming step for forming a first layer (28) on a working electrode (14) and a counter electrode (16) by applying and drying thereon a first coating liquid for the reactive portion containing an oxidation/reduction enzyme, and a second layer forming step for forming a second layer (30) on the first layer (28) by applying and drying thereon a second coating liquid for the reactive portion containing a hydrophilic polymer compound and an electron receptor.</p>
