发明名称 |
Reaction solution for cell-free protein synthesis, method of preparing the same, and protein synthesis method using the same |
摘要 |
The present invention provides a cell-free protein synthesis method characterized by a cell-free protein synthesis reaction solution that is weakly reducing and suitable for protein folding, performing a weakly reducing synthesis reaction, and preferably performing a translation reaction with the further addition of a substance catalyzing a disulfide bond exchange reaction at the beginning of the translation reaction. This method allows for proper formation of intramolecular disulfide bonds in the protein and efficiently provides protein having substantially the same function as the original protein. Specifically, it provides antibody protein that binds specifically to an antigen.
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申请公布号 |
US7273615(B2) |
申请公布日期 |
2007.09.25 |
申请号 |
US20040506127 |
申请日期 |
2004.11.04 |
申请人 |
CELLFREE SCIENCES CO., LTD. |
发明人 |
ENDO YAETA;KAWASAKI TAKAYASU;SAWASAKI TATSUYA |
分类号 |
A61K36/00;A61K38/00;C07K16/12;C12P21/00;C12P21/02 |
主分类号 |
A61K36/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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