IMMOBILISIERTE BIOKATALYSATOREN FÜR DIE HERSTELLUNG VON NATÜRLICHEN NUKLEOSIDEN UND MODIFIZIERTEN ANALOGEN DURCH ENZYMATISCHE TRANSGLYKOSYLIERUNGSREAKTIONEN

摘要

The preparation of novel biocatalysts is described; the biocatalysts are produced by the co-immobilization of the recombinant enzymes uridine phosphorylase and purine nucleoside phosphorylase by means of covalent bonds on solid substrates functionalized with epoxy groups. The novel biocatalysts are usable for successive reaction cycles, are resistant to heat and to the presence of solvents, and can advantageously be used in the industrial production of natural nucleosides and of modified analogues of pharmaceutical interest.