| 摘要 |
A two-stage assay for detecting Rhodococcus equi in a DNA containing sample, said method comprising in a first stage, detecting in said sample, the presence or absence of a nucleic acid which is characteristic of a first gene from Rhodococcus equi, which is conserved in all strains of R. equi, and, if this is found to be present, subjecting the sample to a second stage in which the presence or absence of a nucleic acid which is characteristic of a second gene Rhodococcus equi which is specific to a specific host, is detected. The first nucleic acid is suitably quantitated to provide a useful means for determining the amounts of R. equi in the sample. |
