发明名称 |
METHOD FOR SYNTHESIS OF SUPPRESSOR TRNA, DNA CONSTRUCT, AND PRODUCTION OF PROTEIN HAVING NON-NATURAL AMINO ACID INTEGRATED THEREIN BY USING THE DNA CONSTRUCT |
摘要 |
Disclosed is a DNA construct comprising: a non-eukaryotic suppressor tRNA ge ne which contains no internal promoter sequence capable of functioning in an eukaryotic cell; and an eukaryotic or bacteriophage-derived promoter ligated to the 5'-terminus of the tRNA gene. Also disclosed is a method for synthesi s of suppressor tRNA using the DNA construct. Further disclosed is a method fo r production of a protein having a non-natural amino acid integrated therein b y using the DNA construct.
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申请公布号 |
CA2648009(A1) |
申请公布日期 |
2007.09.07 |
申请号 |
CA20072648009 |
申请日期 |
2007.02.22 |
申请人 |
RIKEN |
发明人 |
SAKAMOTO, KENSAKU;YOKOYAMA, SHIGEYUKI;HINO, NOBUMASA;MUKAI, TAKAHITO;KOBAYASHI, TAKATSUGU |
分类号 |
C12N15/79;C12N1/15;C12N1/19;C12N5/10;C12N15/09;C12P21/00 |
主分类号 |
C12N15/79 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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