| 摘要 |
An antibacterial, antifungal and anti-protozoal antibiotic A28829 is produced by growing the strain Streptomyces antibioticus A28829 (NRRL 3207) or a mutant thereof in an aqueous nutrient medium containing an assimilable source of carbon, nitrogen and inorganic salts under aerobic, and preferably submerged aerobic, conditions. The antibiotic is isolated from the mycelium before or after separation from the fermentation broth by extraction with a solvent such as aqueous acetone or ethyl acetate (A). The acetone solution is freed from aceton and the residue is treated with NaCl and ethyl acetate, concentrated, dissolved in petroleum ether and then extracted with methanol. Alternatively, the ethyl acetate extract (A) may be washed with acid, alkali and water and dried. It is purified by counter-current distribution in the system carbon tetrachloride/methanol/water. Further purification may be by chromatography on silica gel followed by elution with ethyl acetate. Antibiotic A28829 is a lipophilic, neutral, colourless crystalline substance, soluble in methanol, ethanol, acetone, ethylacetate, chloroform, ether and petroleum ether; melting point 223 DEG to 227 DEG C.; elementary analysis C = 60.53%, H = 8.52%, N = 1.89%, B = 1.35% and O = difference; molecular weight 868; infra-red spectrum, Fig. 1; nuclear magnetic resonance spectrum (Fig. 2, not shown); U.V. no-maximum between 210 and 400 mm ; thin-layer chromatogram on silica gel, eluant ethyl acetate, Rf = 0.75. A pharmaceutical composition for human and animal administration comprises antibiotic A28829 and a carrier for oral, rectal or parenteral use. It may be added to animal foods and may be used as a general disinfectant, preservative or plant fungicide.ALSO:An antibacterial, antifungal and antiprotozoal antibiotic p A28829 may be used as a disinfectant or preservative for combatting plant fungi
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