AN EXTRACTION METHOD OF A EFFECTIVE COMPONENT IMPROVING AN ACNE FROM AGRIMONIA PILOSA LEDEBOUR AND A COMPOSITION OF COSMETIC INCLUDING THE COMPONENT

摘要

An extraction method of an effective ingredient having acne-improving effects from Agrimonia pilosa Ledebour is provided to maximize extraction efficiency of Agrimonia pilosa Ledebour extract and improve acne safely by using the single extract of Agrimonia pilosa Ledebour without addition of other natural materials. An extraction method of an effective ingredient having acne-improving effects from Agrimonia pilosa Ledebour comprises the steps of: preparing Agrimonia pilosa Ledebour by drying it in the shade and pulverizing the dried Agrimonia pilosa Ledebour; firstly extracting the Agrimonia pilosa Ledebour with 5-20 ml/g of an extraction solvent selected from anhydrous methanol, hydrous methanol, anhydrous ethanol and hydrous ethanol at room temperature for 1-7 days; removing solvent from the first extract of Agrimonia pilosa Ledebour, solubilizing the first extract in distilled water, adding hexane into the extract-containing solution, collecting a hexane layer containing effective ingredient and removing hexane from the hexane layer; and secondly extracting the hexane with fraction supercritical fluid extraction using carbon dioxide at 38-44 deg.C and 200kg/cm^2. The cosmetic composition for improving acne comprises 0.001-20 wt.% of the hexane fraction or secondary extract of Agrimonia pilosa Ledebour.