| 摘要 |
A method for converting non-human antibodies into "humanized" versions having increased safety and efficacy employs a putative ancient binary genetic code to derive new antibody structures. The method entails determining the amino acid sequence of variable regions of heavy and/or light chains of the non-human antibody, identifying the framework sequences of the variable regions and conjoining them into a single heuristic sequence, which is then converted into a binary string equivalent. The binary string is searched in a binary version of a human protein database for a closest match to identify a reference sequence. The framework sequence of the non-human antibody is optionally modified to be identical with the reference sequence, then reassembled with complementarity-determining regions to produce a full-length heavy and/or light chain template. A DNA segment encoding the template is synthesized and expressed to afford the humanized version of the non-human antibody.
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