发明名称 |
Purified active HCV NS2/3 protease |
摘要 |
A method for producing a refolded, inactive form of recombinantly produced NS2/3 protease which comprises the steps of: a) purifying the protease from inclusion bodies in the presence of a chaotropic agent; and b) refolding the purified protease by contacting it with a reducing agent and lauryldiethylamine oxide (LDAO) in the presence of reduced concentration of chaotropic agent or polar additive. The invention further comprises a method for activating this refolded inactive NS2/3 protease by adding an activation detergent. This method produces large amounts of the active NS2/3 protease to allow small molecules and ligands to be screened as potential inhibitors of NS2/3 protease, which may be useful as therapeutic agents against HCV.
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申请公布号 |
US7264811(B2) |
申请公布日期 |
2007.09.04 |
申请号 |
US20030650585 |
申请日期 |
2003.08.28 |
申请人 |
BOEHRINGER INGELHEIM (CANADA) LTD. |
发明人 |
LAMARRE DANIEL;PILOTE LOUISE |
分类号 |
A61K39/29;G01N33/50;C07K14/18;C12N5/02;C12N7/01;C12N9/50;C12N15/50;C12N15/57;C12P21/00;C12Q1/37;G01N33/15;G01N33/68 |
主分类号 |
A61K39/29 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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