PRODUCTION METHOD OF CYST EXPRESSED TRANSGENIC ANIMAL USING PKD2 GENE

摘要

A production method of a cyst expressed transgenic animal using PKD2 gene is provided to obtain a production method of an animal that expresses cyst only by the over expression of the PKD2 gene. A production method of a cyst expressed transgenic animal using PKD2 gene includes: a first step of manufacturing PKD2 protein expressing vector; a second step of producing a fertilized egg by inserting the expressed vector into the nucleus of the fertilized egg; and a third step of producing transgenic animal by planting the produced fertilized egg into the womb of a surrogate mother. A plasmid is cut by using restriction enzyme Xbal and Xhol(Promega) and introducing PKD2 gene to prepare the expression vector.