The invention relates to a sensor for indicating the transduction and inhibition of stress signals through the p38-MAPK pathway in living cells. The sensor comprises a reporter gene product and an isoform of p38 Mitogen Activated Protein Kinase (MAPK). The invention also provides plasmid and viral vectors containing nucleic acids encoding the sensor for the transfection of living cells. Stable cell lines expressing the sensor can be used in a live-cell or fixed-cell assay to measure activation or modulation of the pathway.
申请公布号
WO2007094867(A2)
申请公布日期
2007.08.23
申请号
WO2006US60251
申请日期
2006.10.26
申请人
GE HEALTHCARE UK LIMITED;STUBBS, SIMON L. J.;HATHER, CATHERINE;ROSS, SANDRA;POWERS, DAVID
发明人
STUBBS, SIMON L. J.;HATHER, CATHERINE;ROSS, SANDRA;POWERS, DAVID