Primers useful in polymerase chain reaction for the identification of subtypes C1, C2 and C3 of staphylococcal enterotoxin type C

摘要

The invention provides primers designed on base of the difference of sequences among staphylococcal enterotoxin subtypes, i.e., C₁, C₂ and C₃. The invention also provides a PCR method for detecting subtypes of staphylococcal enterotoxin type C by using the above-mentioned primers. The invention relates also to DNA probes useful for the identification of subtypes C₁, C₂ and C₃ of staphylococcal enterotoxin type C in various food and clinical samples. The primers of the invention comprises following sequences: ENTC1 5'-ACAGA GTTAT TAAAT GAAGG-3'; ENTC2 5'-GTATC AGCAA CTAAA GTTAT-3'; ENTC3 5'-AAGAG ATTAT TTATT TCACGT-3'; ENTCR 5'-ATCAT ACCAA AAAGT ATTGC-3'.