| 摘要 |
Mammary gland-specific human erythropoietin expression vectors, a transgenic animal and a method for producing human erythropoietin by using the same vector and animal are provided to express high concentration of erythropoietin in milk, and improve stability and physiologically activating effects of the produced human erythropoietin. The mammary gland-specific human erythropoietin expression vector, pBC1-hEPO-WPRE, contains a DNA sequence of beta-casein promoter of SEQ ID NO:2 as a mammary gland-specific promoter, a DNA sequence encoding human erythropoietin of SEQ ID NO:1 and WPRE(woodchuck hepatitis virus post-transcriptional regulatory element) DNA sequence of SEQ ID NO:3. The method for producing human erythropoietin comprises the steps of: transforming an animal selected from pig, mouse, cow, goat, sheet and chicken with the mammary gland-specific human erythropoietin expression vector; and isolating and purifying the human erythropoietin expressed in milk produced from the transgenic animal.
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