发明名称 |
High sensitivity immunoassay method |
摘要 |
A method for detecting a cytokine in a biological fluid sample with a high sensitivity is provided. A time-resolved fluoroimmunoassay (TR-FIA) method including a step of forming on a solid phase a composite in which a cytokine is captured and which includes a fluorescent structural portion which has been complexed with a lanthanoid metal ion, and measuring fluorescence of the fluorescent structural portion. The composite is formed of a structure in which (a) a first antibody including a portion bound to a solid phase and a region bindable to a cytokine; (b) the cytokine; (c) a second antibody including a region bindable to the cytokine and a portion to which biotin is bound; (d) a conjugate including streptoavidin or avidin and a fluorescent structural portion capable of being complexed with a lanthanoid metal ion; and (e) the lanthanoid metal ion are bound. The fluorescent structural portion is represented by General Formula (I): R-Ar-C(-O)-CH<SUB>2</SUB>-C(-O)-C<SUB>n</SUB>F<SUB>2n</SUB>-X.
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申请公布号 |
US7255998(B1) |
申请公布日期 |
2007.08.14 |
申请号 |
US20000089776 |
申请日期 |
2000.09.28 |
申请人 |
JAPAN SCIENCE AND TECHNOLOGY CORPORATION |
发明人 |
TASHIRO KEI;HONJO TASUKU;IKEGAWA MASAYA;MATSUMOTO KAZUKO |
分类号 |
G01N33/53;C07K1/10;G01N21/76;G01N33/24;G01N33/532;G01N33/533;G01N33/547;G01N33/58;G01N33/68 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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