METHOD FOR SIMULTANEOUS DNA TESTING TO DETECT PRESENCE OF H63D AND C282Y POLYMORPHISMS IN HFE GENE ASSOCIATED WITH GENETIC HEMACHROMATOSIS

摘要

FIELD: molecular biology, medicine. ^ SUBSTANCE: disclosed is improved method for simultaneous genetic typing of H63D and C282Y polymorphisms in HFE gene by using PCR method in real time with fluorescent detection. Claimed method includes a) step of reaction mixture containing no analyzed DNA preincubation at +4°C for at least 1 hour; b) application of "hot start" method to activate reaction and c) selection of optimal pairs of fluorescent dye/quenching agent, namely FAM and BHQ1 for normal variant 63H probe; R6G and BHQ1 for mutant variant 63D probe; ROX and BHQ2 for normal variant 282C probe; and Cy5 and BHQ3 for mutant variant 282Y probe. Claimed method is fully adapted to clinical application. ^ EFFECT: method with high specificity level. ^ 10 dwg, 2 tbl 3 ex