PROCESS FOR THE PREPARATION AND ISOLATION OF PHOSPHATIDES

摘要

Process for the preparation of phosphatidylserine of formula CH₂OR¹ CHOR² CH₂O-P(=O)-OCH₂-CH(NH₂)-COOH X wherein R₁ and R₂ independently represent a saturated, mono-unsaturated or polyunsaturated acyl C₁₀-C₃₀, X=OH or OM where M=alkaline or alkaline earth metal, ammonium, alkylammonium (including the inner salt) including the transphosphatidylation reaction between phosphatidylcholine of the general formula CH₂OR¹ CHOR² CH₂O-P(=O)-OR³ X wherein R¹ and R² and X have the above specified meanings, R³=CH₂-CH₂- NH₂ or CH₂-CH₂-N⁺(CH₃)₃ and Serine in D, L or racemic form catalysed hy the phospholipase D enzyme (PLD), characterised in that said reaction is carried out in a hydroalcoholic medium containing an aliphatic alcohol and in the presence of bivalent metal oxide.