发明名称 |
Microbial strain useful for producing recombinant proteins comprises a gene coding for a recombinant protein and a gene that codes for a host protein and is mutated to reduce expression of the host protein |
摘要 |
<p>New microbial strain comprises a gene coding for a recombinant protein that is secreted during fermentation and a mutated gene that codes for a host protein and has a mutation such that expression of the host protein is reduced. Independent claims are also included for: (1) producing a microbial strain as above by transforming a host cells with the gene for the recombinant protein, culturing the cell in a fermentation medium so that the recombinant protein and host proteins are secreted into the medium, characterizing the host proteins, and reducing or preventing expression of a gene coding for one of the host proteins; (2) producing a recombinant protein by culturing a microbial strain as above in a fermentation medium and then separating the medium from the cells of the microbial strain.</p> |
申请公布号 |
DE102006004871(A1) |
申请公布日期 |
2007.08.09 |
申请号 |
DE20061004871 |
申请日期 |
2006.02.02 |
申请人 |
WACKER CHEMIE AG |
发明人 |
LEONHARTSBEGER, SUSANNE;WICH, GUENTER |
分类号 |
C12N1/21 |
主分类号 |
C12N1/21 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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