摘要 |
The invention relates to apparatus, materials and methods for isolating RNA or DNA from a sample . The apparatus comprises magnetic elevator means for separating magnetic particles. Aspirating and dispensing pipettes are provided, and an automated isolation method involving washing, elution, and lysis is disclosed. A lysis solution for lysing cells comprises an alkali metal salt at a concentration of about 2 M to about 10 M, a detergent at a concentration of 0.05 to 2.0% w/v, a surfactant at a concentration of about 5 to 40% w/v, a chelating agent at a concentration of about 5 to 20 mM, an antifoaming agent, a buffer, and antifoam agent of about 0.005% to about 0.1%, wherein the solution has a pH of at least 7. |
申请人 |
GENTRA SYSTEMS, INC.;QUINN, TIM;WIRBISKY, ALAN;VAN DER LUGHT, NICK;MORKEN, NATE;JOHNSON, REBECCA;PAULSEN, KIM;STROM, DAN;BALDRICA, JIM |
发明人 |
QUINN, TIM;WIRBISKY, ALAN;VAN DER LUGHT, NICK;MORKEN, NATE;JOHNSON, REBECCA;PAULSEN, KIM;STROM, DAN;BALDRICA, JIM |