| 摘要 |
Provided is an operation controller of a culture tank which can more precisely control the internal state of the culture tank by specifying a relatively small number of internal state variables, thus constructing a mathematical model which describes intracellular conditions by using these internal state variables, and thereby incorporating observable external variables thereto. The operation controller includes: a culture tank in which a culture medium for culturing animal cells or microorganism is enclosed, a measuring device for measuring concentrations of nutrient components, concentrations of products, and the number density of cells in the culture medium; a supply device for replenishing the nutrient components and oxygen into the culture medium; and an arithmetic processing unit in which the measured values from the measuring device are inputted, and which thus controls the supply device. The arithmetic processing unit solves an equation derived from a circuit network of intracellular reaction rates stored in a storage device by using temporal rates of change of the concentrations of the nutrient components, of the concentrations of the products, and of the number density of the cells as input data, and thus calculates a desired intracellular reaction rate. Accordingly, the arithmetic processing unit controls the supply device based on the results of the calculation, whereby the arithmetic processing unit controls the concentrations of the components contained in the culture medium.
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