THE DNA CHIP FOR THE DETECTION OF SPECIFIC REACTION OF HUMAN FLORA AND THE DETERMINING METHOD OF HUMAN DISEASES USING THE SAME

摘要

A DNA chip is provided to evaluate the harm to human body caused by changing of human flora, which is prepared by identifying and selecting 90 kinds of genes which significantly react to the change of the human flora in colon crypt cell of human flora-associated mice and amplifying and purifying the genes. The DNA chip for detecting specific reaction of human flora comprises at least one gene selected from a gene table(A) attached to a substrate, wherein the gene table(A) consists of Dap3, Rpa1, Ccnd2, Cdc45l, Gmnn, Cul4b, Tacc2, Bnip3l, Slc26a1, Ddx1, Cacnb3, Rpl27, Slc22a1l, Cav, Tuba4, Lrp10, Mt-1, Tiam2, Zdhhc3, Rhcg, Ipo4, 2610529I12Rik, Igj, Daf1, Il18, Tnfsf13b, Prkiri, Serping1, Eif2ak3, Mpp1, Lnx1, 4732481h14Rik, Rgs12, Dcamkl1, Mllt1, Per1, Keap1, Hdac5, Ncoa6, Crem, Crsp7, Rnf12, Alas1, Cotl11, Gstm2, Siat9, 5430437P03Rik, Purb, Col3a1, Il16, Mut, 6330590F17Rik, Srpk2, Klf3, Cited1, D230019K24Rik,, Ugcg, Au043625, Rw1-pending, Hsd17b2, 5031404N19, Tccr, Npm1, Sh3glb2, Aldh6a1, Plp, Ptgs1, Fads3, Parva, C130039O16, Epc1, CPd, Mtmr7, 4930455F23Rik, Rab6ip1, Mcpt4, Fn3k, 1110037F02Rik, 1110038M16Rik, 170012H12H17Rik, Thsd1, 98301480O20Rik, Ptgs2, Tcp11, Guca1a, Usp15, Ybx2, Tcl1, Cldn8 and Ckap2. The method comprises the steps of: (a) reacting the DNA chip with RNA which is collected from colon crypt cells and amplified; and (b) investigating the gene expression degree of the DNA chip.