Identification Of A Nitrilase From B. Japonicum By Rational Genome Mining And Methods Of Use

摘要

The present disclosure relates to methods of rational genome mining. A method may include narrowing the number of clones that would otherwise need to be screened and/or identifying a gene with a desired catalytic activity. The disclosure also relates to a nitrile hydrolase from Bradyrhizobium japonicum USDA110 first identified by rational genome mining. In addition, the disclosure relates to nitrilase bll6402 and catalytically active variants capable of converting an alpha-hydroxy nitriles, a beta-hydroxy nitrile and/or an alpha,omega-dinitrile to a carboxylic acid.