发明名称 |
Cloning, sequencing and expression of a Comamonas cyclopentanone 1,2-monooxygenase-encoding gene in escherichia coli |
摘要 |
Cyclopentanone 1,2-monooxygenase (CPMO) from Comamonas (previously Pseudomonas) sp. strain NCIMB 9872 carries out the second step of a degradation pathway that allows the bacterium to use cyclopentanol as a sole carbon source for growth. In the present invention there is reported the localization of the CPMO-encoding gene (cpnB) on a 4.3-kb SphI fragment, the determination of its sequence. The 550-amino acid CPMO polypeptide (M<SUB>r</SUB>, 62,111) encoded by the gene was found to have 36.5% identity with the sequence of cyclohexanone 1,2-monooxygenase (CHMO) of Acinetobacter sp. strain NCIMB 9871. The 62-kDa CPMO was expressed in E. coli as an IPTG-inducible protein.
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申请公布号 |
US2007178558(A1) |
申请公布日期 |
2007.08.02 |
申请号 |
US20070727730 |
申请日期 |
2007.03.28 |
申请人 |
NATIONAL RESEARCH COUNSIL OF CANADA |
发明人 |
IWAKI HIROAKI;HASEGAWA YOSHIE;LAU PETER C. |
分类号 |
C07H21/04;C12N15/09;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12N9/02;C12N15/53;C12N15/74;C12P21/06 |
主分类号 |
C07H21/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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