METHOD FOR SPECTROSCOPY OF SURFACE PLASMONS IN SURFACE PLASMON RESONANCE SENSORS AND ELEMENT FOR THE USE OF THEREOF

摘要

The method of spectroscopy of surface plasmons consisting in that electromagnetic wave is made incident on a diffraction grating on which surface plasmons excitation and dispersion of wavelength spectrum of the electromagnetic wave are simultaneously performed through diffraction. Changes in spatial distribution of intensity in the wavelength spectrum of the diffracted electromagnetic radiation due to the excitation of surface plasmons are measured. The spectroscopy of surface plasmons can be simultaneously performed in at least two diffraction gratings or different areas of a single grating using at least two electromagnetic waves or different parts of a single electromagnetic wave. The electromagnetic wave can be emitted from at least two monochromatic or a single polychromatic source of electromagnetic radiation. The characteristic of the wavelength-dispersed diffracted electromagnetic radiation is usually detected with at least two individual detectors, a linear array of detectors or two-dimensional array of detectors. The sensor element (1) of a multichannel SPR sensor encompasses one or multiple diffraction gratings which are coated or partially coated with a metal layer (7) for excitation of surface plasmons (2). At least one sensing area (12) of the sensor element (1) is fully or partly coated with a layer containing selected molecules for study of molecules and their interaction or for detection of chemical or biological substances.