发明名称 |
Assays for detection of bioactive compounds that interact with heat shock protein 90 |
摘要 |
A method for evaluation of molecules to identify those that can act as therapeutic inhibitors of Hsp90 makes use of a fluorescence polarization (FP) assay and a cell based assay, either individually or in combination. The FP assay uses a fluorescently-labeled Hsp90 binding agent and measure the degree of fluorescence polarization relative to the standard. A decrease in the degree of polarization indicates that fluorescently-labeled Hsp90 binding agent has been wholly or partially displaced by a candidate molecule, and identifies the molecule as having activity as an inhibitor of Hsp90. The cell based assay tests for decrease is an Hsp90-dependent activity of normal or tumor cells.
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申请公布号 |
US2007178537(A1) |
申请公布日期 |
2007.08.02 |
申请号 |
US20040595029 |
申请日期 |
2004.06.30 |
申请人 |
SLOAN-KETTERING INSTITUTE FOR CANCER RESEARCH |
发明人 |
CHIOSIS GABRIELA;ROSEN NEAL;HUEZO HENRI |
分类号 |
G01N33/574;C07K16/46;C12N;G01N21/21;G01N33/52;G01N33/53;G01N33/566;G01N33/567 |
主分类号 |
G01N33/574 |
代理机构 |
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地址 |
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