METHOD OF CONSTRUCTING AMINO ACID PRODUCING BACTERIA, AND METHOD OF PREPARING AMINO ACIDS BY FERMENTATION WITH THE CONSTRUCTED AMINO ACID PRODUCING BACTERIA

摘要

A METHOD OF PRODUCING CORYNEFORM BACTERIA HAVING AN IMPOVED AMINO ACID-OR NUCLEIC ACID-PRODUCTIVITY COMPRISES THE STEPS OF CAUSING A MUTATION IN A PROMOTER SEQUENCE OF AMINO ACID-OR NUCLEIC ACID-BIOSYNTHESIZING GENES ON A CHROMOSOME OF A CORYNEFORM BACTERIUM TO MAKE IT CLOSE TO A CONSENSUS SEQUENCE OR INTRODUCING A CHANGE IN A PROMOTER SEQUENCE OF AMINO ACID-BIOSYNTHESIZING GENES ON A CHROMOSOME OF A CORYNEFORM BACTERIUM BY THE GENE RECOMBINATION TO MAKE IT CLOSE TO A CONSENSUS SEQUENCE, TO OBTAIN MUTANTS OF THE CORYNEFORM AMINO ACID-OR NUCLEIC ACID-PRODUCING MICROORGANISM, CULTURING THE MUTANTS AND SELECT A MUTANT CAPABLE OF PRODUCING THE INTENDED AMINO ACID OR NUCLEIC ACID IN A LARGE AMOUNT. THIS METHOD CAN CONSTRUCT A MUTANT CAPABLE OF SUITABLY ENRICHING OR CONTROLING THE EXPRESSION OF AN INTENDED GENE WITHOUT USING A PLASMID AND ALSO CAPABLE OF PRODUCING AMINO ACIDS INA HIGH YIELD, BY THE RECOMBINATION OR MUTATION.