发明名称 |
Asymmetrical adapters and methods of use thereof |
摘要 |
A pair of asymmetrical, partially double-stranded oligonucleotide adapters are provided wherein the pair of adapters comprise a first asymmetrical oligonucleotide adapter comprising a single-stranded 3' overhang and a second asymmetrical double-stranded oligonucleotide adapter comprising a single-stranded 5' overhang and at least one blocking group on the strand of said second asymmetrical oligonucleotide adapter that does not comprise the 5' overhang. Also provided are a pair of double-stranded Y oligonucleotide adapters and a pair of double-stranded bubble oligonucleotide adapters and methods of using said asymmetrical adapters for amplification of at least one double stranded nucleic acid molecule, wherein the amplification produces a plurality of amplified nucleic acid molecules having a different nucleic acid sequence at each end are also described. Also provided is a method for exponentially amplifying one strand in a double-stranded nucleic acid molecule. Also provided are methods for preparing libraries of paired tags using COS-linkers. Also provided are cleavable adapters comprising an affinity tag and a cleavable linkage, wherein cleaving the cleavable linkage produces two complementary ends. Methods of using the cleavable adapters to produce a paired tag library are also described.
|
申请公布号 |
US2007172839(A1) |
申请公布日期 |
2007.07.26 |
申请号 |
US20060338620 |
申请日期 |
2006.01.24 |
申请人 |
SMITH DOUGLAS R;MALEK JOEL A |
发明人 |
SMITH DOUGLAS R.;MALEK JOEL A. |
分类号 |
C12Q1/68;C07H21/04;C12P19/34 |
主分类号 |
C12Q1/68 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|