| 摘要 |
1,241,750. Preparing daunorubicin. RHONEPOULENC S.A. 17 Nov., 1969 [18 Nov., 1968], No. 56210/69. Heading C2A. The antibiotic daunorabicin is prepared by aerobically cultivating Streptomyces bifurcus, strain D.S. 23,219, NRRL 3539, or a daunorubicin-producing mutant thereof, on a nutrient medium containing assimilable sources of carbon, nitrogen and inorganic substances, and separating daunorubicin from the culture medium. The preferred culture conditions are pH 6À0 to 7À8 at 23‹ to 33‹ C. The daunorubicin may be separated by extracting the fermentation broth with water-immiscible solvent (e.g. butanol, ethyl acetate or chloroform) at a pH between 5À5 and 9, or preferably by filtering the broth either acidified to pH 1À5 to 2À0 with oxalic acid or admixed with a C 1 -C 3 , aliphatic alcohol and then extracting the filtrate with a water-immiscible solvent. The antibiotic may be recovered by evaporating the solvent or by addition of a non-solvent. The daunorubicin may be converted to its acid-addition salt, e.g. the hydrochloride. Antibiotics 13213 R.P. and 13330 R.P. are also produced in the culture but are not isolated. |
