Embodiments of the present invention are directed to apparatus and methods for performing separations of compounds held in a solution. Embodiments of the present invention allow detectors to operate at constant reproducible temperatures that are lower than the temperature of fluids exiting separation devices. An apparatus of the present invention has pump means, fluid conveying means, sample injection means, separation means and heat dissipation means. The heat dissipation means is in fluid communication with the separation means for receiving the fluid and removing thermal energy.
