METHOD FOR ANALYZING PROTEIN-PROTEIN INTERACTION

摘要

A method for analyzing the interaction between protein and protein is provided to rapidly and conveniently analyze in vivo protein-protein interaction by using a vector which is able to perform bimolecular fluorescence complementation(BiFC) as well as portion-specific recombination. The method comprises the steps of: (a) introducing a nucleic acid coding a target protein or a nucleic acid coding a protein to be analyzed respectively into a vector expressing in a fusion protein form fused with an N-terminal region of a fluorescent protein and a vector expressing in a fusion protein form fused with a C-terminal region of a fluorescent protein, wherein the vector includes a nuclear localization signal at the lower portion of the promoter, and portion-specific recombination including a DNA recombination sequence pair for DNA sequence exchange occurs in the vector; (b) transforming the vector expressing the target protein and the vector expressing the protein to be analyzed into a cell and culturing it; and (c) measuring the interaction between the target protein and the protein to be analyzed through the fluorescence of the fluorescent protein, wherein the fluorescent protein is yellow fluorescent protein(YFP), green fluorescent protein(GFP), enhanced GPF, blue fluorescent protein(BFP) or cyan fluorescent protein(CFP).