A PROCESS FOR ISOLATING VASCULAR ENDOTHELIAL CELLS FROM EMBRYOID BODIES DIFFERENCIATED FROM EMBRYONIC STEM CELLS

摘要

A process for isolating vascular endothelial cells from embryoid bodies differentiated from embryonic stem cells is provided to rapidly and efficiently recover the differentiated embryoid bodies by selectively removing portions having a small amount of the vascular endothelial cells, and minimize cell damage by using low concentration enzyme solution. The process for isolating vascular endothelial cells from an embryoid body differentiated from embryonic stem cells comprises the steps of: (a) treating the embryoid body differentiated from the embryonic stem cell with 0.005-0.015% of trypsin and 0.05-0.15 mM of ethylenediamine tetraacetate(EDTA) for 3-10 minutes; and (b) treating the vascular endothelial cells obtained from the step(a) with 0.1-0.5% of trypsin and 0.5-2 mM of EDTA for 3-10 minutes.