| 摘要 |
The present invention relates to a keratinocyte growth factor fragment, KGF<SUB>des1-23</SUB>, or an analog thereof that is composed of a portion of an amino acid sequence of mature, full length keratinocyte growth factor, KGF<SUB>163</SUB>. The fragment exhibits at least a 2-fold increase in mitogenic activity as compared to a mature, recombinant keratinocyte growth factor, rKGF, but lacks a sequence comprising the first 23 amino acid residues, C-N-D-M-T-P-E-Q-M-A-T-N-V-N-C-S-S-P-E-R-H-T-R- (SEQ ID NO: 2) of the KGF<SUB>163 </SUB>N-terminus. The present invention also relates to a DNA molecule encoding KGF<SUB>des1-23</SUB>, an expression vector and a transformed host containing the DNA molecule, and a method of producing KGF<SUB>des1-23 </SUB>by culturing the transformed host. The present invention further relates to a conjugate of KGF<SUB>des1-23 </SUB>and a toxin molecule, and the use thereof for treatment of hyperproliferative disease of the epidermis. Moreover, the present invention relates to a therapeutic composition containing KGF<SUB>des1-23 </SUB>and a pharmaceutically acceptable carrier and the use thereof for wound healing purposes.
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