Chromatographic separation method, separation device and process for the preparation of a separation medium for use therein

摘要

A method for the chromatographic separation of substances contained in a liquid sample is disclosed which method comprises providing a one piece separation tray having a spaced array of discrete identical upstanding chambers each exhibiting an open upper end and an open lower end and a separation medium placed in at least part of each upstanding chamber; applying a liquid sample to said open upper end of at least one of said upstanding chambers; then applying an eluting liquid to said open upper end of said at least one of said upstanding chambers; and collecting at least one product fraction flowing out from the open lower end of said at least one of said upstanding chambers; wherein a monolith of a compressible macroporous gel having in its liquid-swollen, non-compressed state a cross-sectional area which is 2-15%, preferably 4-12% and most preferably 5-10%, larger than the cross-sectional area of the upstanding chamber in which it is placed is used as said separation medium and is in face-to-face contact with the wall of the respective chamber in its liquid-swollen state. Processes for the preparation of monoliths to be used in such a method and a separation device for use in said method are also disclosed.