Chemiluminescent compounds

摘要

The invention relates to chemiluminescent compounds of general formula: (I) wherein: either: R₁ is a reactive group capable of reacting with an amine or thiol moiety; L₁ is a hydrocarbon linker moiety comprising 2-12 carbon atoms, optionally substituted with hydroxy, halo, nitro or C₁-C₄ alkoxy; and R₂is hydrogen, C₁-C₄ alkyl, C₁-C₄ haloalkyl, aryl, fused aryl, C₁-C₄ alkoxy, C₁-C₄acyl, halide, hydroxy or nitro; or, alternatively: the combination R₁-L₁- comprises a C₁-C₄ alkyl group optionally substituted with hydroxy, halo, nitro or C₁-C₄ alkoxy; and R₂ comprises a group R₄-L₁-, where R₄ is a reactive group capable of reacting with an amine or thiol moiety; and L₁ is as defined above; L₂ is -C(-O)O-, -C(-O)-S- or -C(-O)N(SO₂R₅)-, wherein, in each case, the -C(-O) is linked to the ring carbon atom, and R₅ is C₁-C₈ alkyl, aryl, C₁-C₈ alkoxy or C₁-C₈ acyl; R₃ a substituted C₁-C₈ alkyl, C₂-C₈ alkenyl, C₁-C₈ alkynyl or aryl group wherein at least one of the said substituents is electron-withdrawing such that the pKa of the conjugate acid of the leaving group formed from R₃ and the -O, -S or -N(SO₂R₅) of the L₂ group is <= ABOUT 9.5; AND X^- is an anion formed as the result of the synthesis and processing of the molecule; wherein the compound may contain one or more additional R₂ moieties on either or both outer rings, provided that only one of said R₂ moieties may comprise an R₄-L₁-group.