| 摘要 |
PROBLEM TO BE SOLVED: To provide an immuno-labeled complex and a method having applications to the fields of molecular biology, cell biology, immunohistochemistry, diagnostics, and therapeutics and for use of detection and measurement of one target or more in a biological sample. SOLUTION: The method for forming an immuno-labeled complex includes a process (a) of contacting a solution of target-binding antibodies with a labeling reagent subset, wherein the labeling reagent subset is distinguished by (i) a ratio of label to labeling reagent or (ii) a physical properties of the label; a process (b) of incubating the target-binding antibodies and the labeling reagent subset for a time period sufficient for one labeling reagent or more to form an immuno-labeled complex with a target-binding antibody wherein a region of the target binding antibody is selectively bound by the labeling reagent; a process (c) of optionally removing unbound labeling reagents by adding a capture reagent comprising immunoglobulin proteins or fragments thereof; and a process (d) of optionally repeating the processes (a), (b), and (c) to form a panel of immuno-labeled complex subsets. The labeling reagent contains no protain A. COPYRIGHT: (C)2007,JPO&INPIT
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