Apparatus and method for the manipulation and testing of molecules, and in particular of DNA

摘要

An apparatus for manipulating and testing molecules includes a surface to which the molecule is anchored at multiple points at one end, a paramagnetic bead on which said molecule is anchored at multiple points at its other end, and a magnetic means for applying a force to the bead, said magnetic means being used to control the stretching and rotation of said bead and said molecule. The apparatus also includes an optical magnification means and a camera for the visualization of said bead. The apparatus further includes a computer means to which images from the camera are transmitted. The computer means includes means for analyzing the motions of the bead. The apparatus also includes a rotating means for spinning the magnetic means about an optical axis of the magnification means. The magnetic means is positioned at a distance from the molecule in the direction of the optical axis of the magnification means, perpendicular to the optical axis, so that the force applied to the bead is substantially directed parallel to said optical axis and the field produced by the magnetic means is perpendicular to the optical axis. Rotation and translation of the magnetic means causes rotation and translation of the bead, which allows to control stretching and torsion of the molecule.