发明名称 ADSORPTION VON NUKLEINSÄUREN AN EINE FESTPHASE
摘要 The present invention is directed to a method for adsorbing, i.e. non-covalently binding, nucleic acids to a solid phase using a two-step procedure. Furthermore, the present invention pertains to a method for isolating nucleic acids from a biological sample. In the first step of the procedure, lysis is effected by mixing the biological sample with an aequous lysis buffer containing a chaotropic agent and incubating the mixture; in the second step, the concentration of the chaotropic agent in the mixture is increased and the mixture is contacted with the solid phase, whereby the nucleic acids in the liquid phase is adsorbed to the solid phase. <IMAGE>
申请公布号 AT364694(T) 申请公布日期 2007.07.15
申请号 AT20050003254T 申请日期 2005.02.16
申请人 F.HOFFMANN-LA ROCHE AG 发明人 ZIELENSKI, RALF;GEISSLER, KLAUS;WALTER, THOMAS
分类号 C12N15/09;C12N15/10;B01J20/00;C07H1/08;C07H21/00;C07H21/02;C07H21/04;C12N1/08;C12Q1/68;G01N33/15;G01N33/53 主分类号 C12N15/09
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