NOVEL MAIZE SPLIT-SEED EXPLANT AND METHODS FOR IN VITRO REGENERATION OF MAIZE

摘要

The present invention provides an efficient and novel maize transformation and regeneration system based on a novel split-seed explant. Mature maize seeds are split longitudinally to form a split-seed explant. The split-seed explant can then be used in transformations to introduce a gene of interest into the maize genome to produce novel maize lines having desired characteristics. The split-seed explant can also be used to generate calli and/or multiple shoots, and rooted plantlets.