| 摘要 |
<p><P>PROBLEM TO BE SOLVED: To provide a simple method having excellent specificity, capable of performing high-throughput analysis, and profiling comprehensively activity in various protein kinases. <P>SOLUTION: This phosphorylation detection method, which is a method for detecting phosphorylation of a peptide or a protein on a substrate, is characterized by performing Michael addition by applying a fluorescent material modified by, for example, a thiol group, afterβ-elimination of a phosphorylated peptide or protein. <P>COPYRIGHT: (C)2007,JPO&INPIT</p> |
