Standardized and optimized real-time quantitative reverse transcriptase polymerase chain reaction method for detection of MRD in leukemia

摘要

The invention relates to a real-time quantitative reverse transcriptase polymerase chain reaction (RQ-PCR) method for minimal residual disease detection in leukemic patients through amplification of a fusion gene transcript BCR-ABL, comprising:
(i) selecting amplifiable and qualified patient samples for subsequent analysis;
(ii) defining optimal conditions for performing the RT reaction;
(iii) defining optimal conditions for RQ-PCR protocol; and
(iv) establishing a standardized procedure for data analysis.