METHOD FOR PRODUCTION OF HUMAN GENE ENGINEERED GLUCAGONE, RECOMBINANT PLASMIDE DNA pER-Gl, ENCODING AUTOCATALITICALLY CLEAVABLE HYBRID PROTEIN FORMING HUMAN GLUCAGONE AND STRAIN OF Escherichia coli ER-2566/pER-Gl AS PRODUCER OF SAID PROTEIN

摘要

FIELD: gene and protein- engineering, medicine, pharmaceutical industry. ^ SUBSTANCE: invention relates to recombinant plasmide DNA pER-Gl is constructed, which provides synthesis of hybrid polypeptide containing human glucagone and intein in Escerichia coli cells. Producer of said hybrid polypeptide is obtained by transformation of E.coli ER2566 with pER-Gl plasmide. Method for production of human recombinant glucagone includes providing and cultivation of hybrid polypeptide producer containing human glucagone and intein followed by isolation and cleavage of said hybrid protein. ^ EFFECT: increased yield of human recombinant glucagone; simplified technology. ^ 3 cl, 3 ex, 3 dwg