METHOD OF IMMUNIZING ANIMAL, COMPOSITION FOR IMMUNIZATION, METHOD OF PRODUCING ANTIBODY, METHOD OF PRODUCING HYBRIDOMA AND METHOD OF PRODUCING MONOCLONAL ANTIBODY

摘要

It is intended to provide a method whereby a humoral immune response can be induced at an elevated efficiency in constructing an antibody against an antigen protein by gene immunization. By administering a fused gene composed of a gene encoding an antigen protein as a whole or a part thereof attached to another gene encoding a chaperonin subunit or a chaperonin subunit ligation, the fused gene is expressed in the animal and thus a humoral immune response to the antigen protein is induced. As an example of the chaperonin, Escherichia coli GroEL is cited. It is also intended to provide a composition for immunization, a method of producing an antibody, a method of producing a hybridoma and a method of producing a monoclonal antibody.