SPECIFIC AND SENSITIVE METHOD FOR AMPLIFYING TARGET SEQUENCE

摘要

It is intended to develop a method for reducing a noise from a non-target gene having a similar sequence and amplifying only a target gene and a novel quantitative method by obtaining a signal from the amplified gene with high accuracy (high sensitivity and high specificity). As a result of intensive studies made by the inventors of the invention, it was found that the object can be achieved by incorporating a third primer in a PCR reaction mixture, thus the invention has been completed.