| 摘要 |
Disclosed herein is an SNP discrimination assay for discriminating base species of an SNP in a specific gene in a sample, which includes the steps of hybridizing four detection probes, which have at least a flanking sequence around an SNP in the gene and are different in base species at a site of the SNP, with a target nucleic acid in the sample, the target nucleic acid having at least the flanking sequence around the SN in the gene, respectively, cleaving three of four double-stranded nucleic acids, which contain noncomplementary base pairs, respectively, at sites of the noncomplementary base pairs, denaturing only the three double-stranded nucleic acids, which have been cleaved at the sites of the noncomplementary base pairs, into single-stranded forms, and detecting one of the four detection probes, the one detection probe still retaining a double-stranded form after the nucleic acid denaturation step.
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