| 摘要 |
A method of increasing aurantio-obtusin content in Cassia seed(Cassiae Semen) and a method of purifying aurantio-obtusin content in the Cassia seed are provided. The health supplementary composition and pharmaceutical composition containing the aurantio-obtusin have antimutagenic activity and are effective in inhibiting plant pathogens. To increase aurantio-obtusin content in Cassia seed, the Cassia seed is treated with a crude enzyme extract from Aspergillus kawachii at about 37deg.C for more than 50min. The aurantio-obtusin in the Cassia seed is purified by the steps of: extracting the Cassia seed having increased aurantio-obtusin content with ethanol; dispersing the extract in water, extracting with a solvent selected from the group consisting of dichloromethane and ethyl acetate and concentrating to produce an organic solvent-soluble fraction; and concentrating the organic solvent-soluble fraction and purifying using chromatography. The chromatography uses a solvent selected from the group consisting of methanol, hexane, ethyl acetate, acetic acid, chloroform, benzene and a mixed solvent of two more components thereof and a carrier selected from the group consisting of silica gel, reverse phase silica gel, high speed liquid and Sephadex. |
